Medium 199, Powder, makes 50 L

Medium 199 is a classical cell culture formulation originally developed for nutritional studies and the cultivation of a variety of mammalian cell types. This version includes Earle’s Salts to support use in CO₂ incubators and is supplied without L-glutamine, allowing researchers to supplement the medium according to specific experimental needs. Provided in powder form, it enables convenient large-scale media preparation.

Applications:

• Culturing endothelial cells, fibroblasts, and certain epithelial cell lines
• Nutritional and metabolic studies
• Customizable cell culture systems requiring glutamine control
• Scalable preparation for research or production environments

This glutamine-free formulation offers flexibility for experiments requiring defined supplementation or enhanced stability.