Minimal Essential Medium (MEM), Sterile Filtered, 500 ml
w/ Earle’s Salts and 20 mM HEPES w/o L-glutamine and NaHCO3
Minimal Essential Medium (MEM) is a classical basal medium designed to support the growth of a variety of mammalian cells. This specialized formulation includes Earle’s salts and 20 mM HEPES buffer to provide enhanced pH stability, especially useful in non-CO₂ environments or during handling outside the incubator. It is supplied without L-glutamine and sodium bicarbonate, allowing users to tailor supplementation according to experimental needs. Sterile filtered to ensure purity and consistency.
Applications:
- Culturing mammalian cells in ambient or low-CO₂ conditions
- Experiments requiring tight pH control outside standard incubators
- Customizable culture systems needing defined glutamine and bicarbonate supplementation
- General research in cell biology, pharmacology, and virology
MEM with Earle’s salts and HEPES, without L-glutamine and sodium bicarbonate, offers flexibility and improved buffering capacity for a range of specialized cell culture applications.