RPMI 1640, Sterile Filtered, 500 ml

RPMI 1640 is a widely used basal medium designed for culturing various mammalian cells, especially hematopoietic and lymphoid cell lines. This formulation includes 25 mM HEPES buffer, which enhances pH stability during handling outside CO₂ incubators. It is supplied without L-glutamine, allowing researchers to customize glutamine supplementation as needed. Sterile filtered to ensure high purity and consistent performance.

Applications:

• Culturing suspension and adherent cells requiring enhanced buffering capacity
• Experiments involving manipulation outside of CO₂ incubators
• Research in immunology, oncology, and hematopoietic cells
• Serum-free and defined media applications requiring flexible glutamine supplementation

RPMI 1640 with HEPES and without L-glutamine provides a stable and adaptable environment for specialized cell culture conditions.

Technical Data Sheet

Material Safety Data Sheet