Iscove's Modified Dulbecco's Medium (IMDM), Sterile Filtered, 500 ml
OPTIONS: w/ stable glutamine, 25 mM HEPES, 1 g/L Glucose, w/o Ca
Iscove’s Modified Dulbecco’s Medium (IMDM) is a highly enriched basal medium formulated to support the growth of a variety of mammalian cells, including hematopoietic and hybridoma lines. This formulation features a stable form of glutamine to reduce degradation and ammonia buildup, 25 mM HEPES buffer for enhanced pH stability during culture, and a moderate glucose concentration (1 g/L) to support cellular energy needs. The medium is prepared without calcium, making it suitable for applications where calcium-sensitive processes are critical. Sterile filtered to ensure high purity and consistency.
Applications:
- Culturing calcium-sensitive cell types
- Hematopoietic stem cell and lymphocyte maintenance
- Hybridoma and monoclonal antibody production
- Experimental conditions requiring pH stability and reduced ammonia toxicity
IMDM with stable glutamine, HEPES buffer, and no calcium provides a tailored environment for demanding and specialized cell culture workflows.